Journal: Nature Communications

Article Title: Complement inhibition by a unique cluster of immunomodulatory outer surface proteins of Borrelia recurrentis

doi: 10.1038/s41467-026-72359-y

Figure Lengend Snippet: A Binding of glu-plasminogen to purified borrelial proteins was determined by ELISA. Chi proteins, HcpA and BBA70 (positive controls) or Vsp1 and BSA (negative control) (5 ng/µl each) were immobilized and incubated with 10 ng/µ glu-plasminogen. Protein-protein complexes were detected by using a polyclonal anti-plasminogen antibody (1:1000). Data of at least ten technical replicates ( n = 10, mean, ±SD) were shown and compared with BSA as negative control. ** p ≤ 0.01, **** p ≤ 0.0001, n.s., no statistical significance, one-way ANOVA with post-hoc Bonferroni multiple comparison test. B – F Dose-dependent binding of glu-plasminogen to three selected Chi proteins. Purified ChiA ( B ), ChiB ( C ), ChiC ( D ), and ChiD ( E ), ChiE ( F ) (5 ng/µl each) were immobilized and incubated with increasing concentrations of glu-plasminogen (0.5 to 2 µM). Binding curve and dissociation constant were calculated via non-linear regression, four-parameter model. Data of three technical replicates ( n = 3, mean, ±SD) are shown. G Degradation of C3b by activated plasminogen bound to Chi proteins. Proteins (10 ng/µl each) immobilized were incubated with plasminogen (10 ng/µl). Thereafter, uPA (25 ng/µl) and C3b (20 ng/µl) were added and C3b degradation products were visualized by Western blotting using a polyclonal anti-C3 antibody (1:1000). n.a., not available. Source data are provided as a Source data file.

Article Snippet: Polyclonal anti-plasminogen antibody was purchased from Acris Antibodies (Herford, Germany).

Techniques: Binding Assay, Purification, Enzyme-linked Immunosorbent Assay, Negative Control, Incubation, Comparison, Western Blot

Journal: World Journal of Gastrointestinal Surgery

Article Title: Ligamentum teres hepatis as a graft for portal and/or superior mesenteric vein reconstruction: From bench to bedside

doi: 10.4240/wjgs.v15.i4.674

Figure Lengend Snippet: Distribution and function of endothelial cells and relative content of collagen fibers, elastic fibers and smooth muscle in ligamentum teres hepatis. A: The ligamentum teres hepatis (LTH) lumen (× 100 magnification); B: Endothelial cells (black arrow, × 400 magnification); C: LTH after Verhoeff-Van Gieson (VVG) staining (× 400 magnification); D: The portal vein (PV) after VVG staining. Elastic fibers (red), collagen fibers (black) and smooth muscle (yellow) (× 400 magnification); E: CD34 expression in LTH endothelial cells (× 400 magnification); F: CD34 expression in PV endothelial cells (× 400 magnification); G: Factor VIII-related antigen (FVIIIAg) expression in LTH endothelial cells (× 400 magnification); H: FVIIIAg expression in PV endothelial cells (× 400 magnification); I: Endothelial nitric oxide synthase (eNOS) expression in LTH endothelial cells (× 400 magnification); J: eNOS expression in PV endothelial cells (× 400 magnification); K: Tissue type plasminogen activator (t-PA) expression in LTH endothelial cells (× 400 magnification); L: Tissue type plasminogen activator expression in PV endothelial cells (× 400 magnification).

Article Snippet: Mouse monoclonal anti-human CD34, rabbit monoclonal anti-human factor VIII-related antigen (FVIIIAg), rabbit polyclonal anti-human endothelial nitric oxide synthase (eNOS), and rabbit polyclonal anti-human tissue type plasminogen activator (t-PA) antibodies were purchased from Santa Cruz Biotechnology (CA, United States).

Techniques: Staining, Expressing